LOCUS       pLV-[hsa-miR-367]        9172 bp    DNA     circular      1-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7093..7953
                     /label=Amp(R)
     promoter        8154..8694
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5698..5932
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6507..6962)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6004..6134
                     /label=SV40 pA
     misc_feature    6135..6348
                     /label=SV40 ori
     misc_feature    5019..5615
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3637
                     /label=intron
     CDS             3684..5009
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3427
                     /label=hsa-mir-367 precursor
BASE COUNT     2180 a      2365 c      2464 g      2163 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg acatgacaaa 
     3121 aataagtgct tccatgtttg agtgtggtgg ttcctaccta atcagcaatt gagttaacgc 
     3181 ccacactgtg tgcagttctt ggctacaggc cattactgtt gctaatatgc aactctgttg 
     3241 aatataaatt ggaattgcac tttagcaatg gtgatggatt gttaagccaa tgacagaatt 
     3301 taaaccacag acttactttg atagcactct taatggtata acttcttctc ccattttatg 
     3361 tctctcttta tgttttttct tatgtttcct ttttgttttc aagagagagc tatcttttag 
     3421 atcgccagct agctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta 
     3481 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt 
     3541 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc 
     3601 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaaaac tacccctcta 
     3661 gagtcgagct accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca 
     3721 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 
     3781 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 
     3841 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 
     3901 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 
     3961 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 
     4021 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 
     4081 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 
     4141 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 
     4201 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 
     4261 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 
     4321 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 
     4381 agctgtacaa ggacccaccg gtcgccacca tgaccgagta caagcccacg gtgcgcctcg 
     4441 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 
     4501 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 
     4561 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 
     4621 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 
     4681 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 
     4741 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 
     4801 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 
     4861 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 
     4921 acgagcggct cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct 
     4981 ggtgcatgac ccgcaagccc ggtgcctgag cggccgcgtc gacaatcaac ctctggatta 
     5041 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 
     5101 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 
     5161 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 
     5221 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 
     5281 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 
     5341 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 
     5401 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 
     5461 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 
     5521 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 
     5581 gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga 
     5641 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 
     5701 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc 
     5761 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 
     5821 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 
     5881 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 
     5941 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 
     6001 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     6061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     6121 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc 
     6181 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     6241 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     6301 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt 
     6361 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 
     6421 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 
     6481 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg 
     6541 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 
     6601 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 
     6661 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 
     6721 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 
     6781 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 
     6841 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 
     6901 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 
     6961 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 
     7021 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 
     7081 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 
     7141 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 
     7201 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 
     7261 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 
     7321 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 
     7381 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 
     7441 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 
     7501 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 
     7561 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 
     7621 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 
     7681 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 
     7741 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 
     7801 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 
     7861 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 
     7921 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 
     7981 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 
     8041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 
     8101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 
     8161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 
     8221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 
     8281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 
     8341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 
     8401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 
     8461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 
     8521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 
     8581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 
     8641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 
     8701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 
     8761 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 
     8821 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 
     8881 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 
     8941 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 
     9001 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 
     9061 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat 
     9121 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gc 
//
